Rabbit antibody to APG16 L1: whole serum

$190.00USD

Catalogue No.:	R-158-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Other Names:	Autophagy-related protein 16-1, APG16-like 1, GENE NAME: ATG16L1
Accession:	A16L1_HUMAN
Description:	APG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within APG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Specificity:	IHC, WB and ELISA confirmed the specificity for ATG16 L1.
Cross-react:	Human, rat. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date:	Six months after purchase
References:	1. Zheng H, et al. DNA Seq. 15:303-305(2004). 2. Clark H.F, et al. Genome Res. 13:2265-2270(2003).
Related items:	R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-144-100, R-159-100, R-157-100, R-156-100, R-161-100, R-160-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100

Images

Rabbit antibody to APG16 L1: whole serum

Confocal microscopy on immunofluorescently detected cytospinned-HL60 cell line. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes.
Eosinophils seem to be stained using Rabbit antibody to APG16 L1 : whole serum (R-158-100).

	Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to APG16 L1: whole serum (R-158-100).
	Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to APG16 L1: whole serum (R-158-100) at a dilution of 1: 100 (ECL).

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