| Catalogue No.: |
R-158-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen. |
| Other Names: |
Autophagy-related protein 16-1, APG16-like 1, GENE NAME: ATG16L1 |
| Accession: |
A16L1_HUMAN |
| Description: |
APG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within APG16 L1 are associated with susceptibility to inflammatory bowel disease type 1. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC, WB and ELISA confirmed the specificity for ATG16 L1. |
| Cross-reactivity: |
Human, rat. Other species not yet tested. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
Six months after purchase |
| References: |
1. Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009
2. Zheng H, et al. DNA Seq. 15:303-305 (2004).
3. Clark H.F, et al. Genome Res. 13:2265-2270 (2003). |
