| Catalogue No.: |
R-138-500 |
| Batch No.: |
See product label |
| Unit size: |
500 µg |
| Antigen: |
A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response. |
| Other Names: |
Autophagy protein 5, APG5-like, APG 5, APG5, Apoptosis-specific protein. GENE NAME: ATG5; APG5L; ASP |
| Accession: |
ATG5_HUMAN |
| Description: |
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family. |
| Produced in: |
NZ white rabbit |
| Purity: |
Protein G purified IgG |
| Applications: |
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. The optimal working dilution should be determined by the end user. |
| Specificity: |
IHC and wb confirmed the specificity for APG5. |
| Cross-reactivity: |
Human, not yet tested in other species. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
Six months after purchase |
| References: |
1. Mizushima, N et al. (2003) Int J Biochem Cell Biol. 35(5), 553-61
2. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
3. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
4. Greenberg JT. Dev Cell. 8(6):799-801. (2005) |
