||ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
||See product label
||A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
||Autophagy-related protein 10; APG10-like; ATG10
||IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||IHC, WB and ELISA confirmed the specificity for ATG10.
||Human. Other species not yet tested.
||Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
||Six months after purchase
||1. Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress.
Bone Marrow Res. 2012;2012:329061.
||1. Wan D. et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729 (2004)
2. Ota T. et al. Nat. Genet. 36:40-45(2004)
3. The MGC Project Team. Genome Res. 14:2121-2127(2004).