| Catalogue No. |
R-137-500 |
| Description |
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family. |
| Batch No. |
See product label |
| Unit size |
500 µg |
| Antigen |
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response. |
| Other Names |
Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L |
| Accession |
ATG12_HUMAN |
| Produced in |
Rabbit |
| Purity |
Protein G purified IgG |
| Applications |
IHC, immunofluorescence, WB. A working concentration of 1-5 µg/ml is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
IHC and WB confirmed the specificity for ATG12. |
| Cross-reactivity |
Human, rat |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| References |
1. Mizushima, N. et al. J. Biol. Chem. 273(51):33889-33892 (1998).
2. Tanida, I. et al. Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002).
3. Tanida, I. et al. J. Biol. Chem. 277(16):13739-13744 (2002). |
