Rabbit antibody to ATG3: whole serum

$190.00USD

Catalogue No.:	R-159-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide corresponding to the N-terminus of human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Other Names:	Autophagy-related protein 3, APG3, APG3-like, hApg3, Protein PC3-96 , GENE NAME: ATG3, APG3, APG3L
Accession:	ATG3_HUMAN ATG3_MOUSE ATG3_RAT ATG3_Saccharomyces cerevisiae ATG3_Macaca mulatta ATG3_Canis familiaris
Description:	FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, WB, immunofluorescence. A dilution of 1:50 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Specificity:	IHC, WB and ELISA confirmed the specificity for ATG3.
Cross-react:	Human, rat. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	Six months after purchase
References:	1. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002). 2. Ota T, et al. Nat. Genet. 36:40-45(2004). 3. Rush J, et al. Nat. Biotechnol. 23:94-101(2005).
Related items:	R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-157-100, R-156-100, R-161-100, R-160-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100

Images

Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
More infromation on the staining:
- Fixation: In acetone for 90 seconds
- Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes.
- Magnification 80X
- No staining was observed using the Pre-immunisation serum
- No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)

	Western blot on mouse brain lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50. Absorbtion with the immunising peptide almost removed the three main bands otherwise visible on the unabsorbed strip.
	Western blot under reduceding condition on 293 cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.
	Western blot under reduceding condition on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.

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