Rabbit antibody to ATG4C (APG4C): whole serum

$190.00USD

Catalogue No.:	R-156-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Other Names:	Cysteine protease ATG4C, Autophagy-related protein 4 homolog C, Autophagin-3, Autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase. GENE NAME: ATG4C; APG4C; AUTL3.
Accession:	ATG4 C_HUMAN ATG4 C_MOUSE ATG4 C_RAT
Description:	FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Specificity:	IHC, ELISA and WB confirmed the specificity for ATG4C.
Cross-react:	Human, rat. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	Six months after purchase
References:	1. Marino G, et al. J. Biol. Chem. 278:3671-3678(2003). 2. Ota T, et al. Nat. Genet. 36:40-45(2004).
Related items:	R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-159-100, R-157-100, R-161-100, R-160-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100

Images

Confocal microscopy on immunofluorescently detected APG4C in HL60 cell line using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Note: More IHC analysis is underway. Please enquire.

	Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100).
	Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100 (ECL).
	Western blot under reduceding condition on Olfactory cell line (Odora) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
	Western blot under reduceding condition on rat liver lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
	Western blot under reduceding condition on 293 cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.

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