| Catalogue No. |
R-138-500 |
| Description |
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family. |
| Batch No. |
See product label |
| Unit size |
500 µg |
| Antigen |
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response. |
| Other Names |
Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP |
| Accession |
ATG5_HUMAN |
| Produced in |
Rabbit |
| Purity |
Protein G purified IgG |
| Applications |
IHC, immunofluorescence, WB (band at 49kDa) . A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
IHC and wb confirmed the specificity for ATG5. |
| Cross-reactivity |
Human, not yet tested in other species. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
Six months after purchase |
| Specific References |
1. Cotán D. et al. (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts FASEB J. 2011
|
| References |
1. Mizushima, N et al. (2003) Int J Biochem Cell Biol. 35(5), 553-61
2. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
3. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
4. Greenberg JT. Dev Cell. 8(6):799-801. (2005) |
