| Catalogue No.: |
R-161-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). |
| Other Names: |
Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like, GENE NAME: ATG7, APG7L |
| Accession: |
ATG7_HUMAN
ATG7_MOUSE
ATG7_RAT
ATG7_Saccharomyces cerevisiae
ATG7_Macaca mulatta
ATG7_Canis familiaris |
| Description: |
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHC, immunofluorescence. A dilution of 1:200 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC, WB and ELISA confirmed the specificity for ATG7. |
| Cross-react: |
Human, rat. Other species not yet tested. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
Six months after purchase |
| References: |
1. Yuan W, et al. Mol. Biol. Cell 10:1353-1366(1999).
2. Tanida I, et al. Biochem. Biophys. Res. Commun. 292:256-262(2002).
3. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002).
4. Mizushima N, et al. Nature 395:395-398(1998).
5. Johnston M, et al. Science 265:2077-2082(1994).
6. Harding T.M, et al. J. Cell Biol. 131:591-602(1995).
7. Kim J, et al. Mol. Biol. Cell 10:1337-1351(1999).
8. Komatsu M, et al. J. Biol. Chem. 276:9846-9854(2001).
9. Klionsky D.J, et al. Dev. Cell 5:539-545(2003).
10. chimura Y, et al. J. Biol. Chem. 279:40584-40592(2004).
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| Related items: |
R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-159-100, R-157-100, R-156-100, R-160-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100 |
Images
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Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Observations:
- No staining was observed using the Pre-immunisation serum
- No staining was observed in HL60 cell line
Note: More IHC analysis is undeway. Please enquire. |
 |
Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1:100 (ECL). |
 |
Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. |
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