| Catalogue No.: |
R-160-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). |
| Other Names: |
Autophagy-related protein 9A, APG9-like 1, Autophagy-related protein 9; APG9A, GENE NAME: ATG9A, APG9L1 |
| Accession: |
ATG9 L1_HUMAN
ATG9 L1_MOUSE
ATG9 L1_RAT
ATG9 L1_Saccharomyces cerevisiae
ATG9 L1_Macaca mulatta
ATG9 L1_Canis familiaris |
| Description: |
FUNCTION: Plays a role in autophagy. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (Potential). ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the ATG9 family. IN YEAST: FUNCTION: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. SUBUNIT: Interacts with ATG18, ATG2 and ATG23. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note=Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. SIMILARITY: Belongs to the ATG9 family. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . |
| Cross-react: |
Human, rat. Other species not yet tested. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
Six months after purchase |
| References: |
1. Ota T, e al. Nat. Genet. 36:40-45(2004).
2. Delaveau T.T.D, et al. Yeast 12:1587-1592(1996).
3. Jacq C, et al. Nature 387:75-78(1997).
4. Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993).
5. Harding T.M, et al. J. Cell Biol. 131:591-602(1995).
6. Harding T.M, et al. J. Biol. Chem. 271:17621-17624(1996).
7. Lang T, et al. J. Bacteriol. 182:2125-2133(2000).
8. Noda T, et al. J. Cell Biol. 148:465-480(2000).
9. Kim H, et al. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
10. Tucker K.A, et al. J. Biol. Chem. 278:48445-48452(2003). |
| Related items: |
R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-159-100, R-157-100, R-156-100, R-161-100, R-141-100, R-142-100, R-143-100, R-146-100, R-155-100, R-140-100 |
Images
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Confocal microscopy on mouse lymph node using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100). |
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Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100). |
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Western blot on Peripheral Blood Mononuclear Cells (PBMC) lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100) at a dilution of 1: 100 (ECL). |
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