Rabbit antibody to BDNF (129-138): affinity purified

$227.00USD

Catalogue No.	R-172-20
Description	BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Batch No.	See product label
Unit size	20 µg
Antigen	A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst many mammalian species.
Other Names	Brain-derived neurotrophic factor; Abrineurin; proBDNF;
Accession	BDNF_HUMAN
Produced in	Rabbit
Purity	Affinity purified
Applications	IHC, ELISA, WB. A working concentration of 1-10 µg/ml is recommended for IHC and ELISA and 0.5-1 µg/ml for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum also easily recognises recombinant human BDNF in western blot.
Cross-reactivity	This antiserum is known to recognise rat, mouse and human BDNF.
Blast it	If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form	Lyophilised
Reconstitution	Reconstitute in 20 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date	12 months after purchase
References	1. A Acheson et al (1995) Nat. 74: 450-3 2. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91 3. XF Zhou et al (1996) Neurosci. 74: 945-53 4. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42 5. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9 6. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107 7. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9 8. Barde Y. A. et al (1989) EMBO J. 1: 549 9. Conner J et al. (1997) J. Neurosci. 17: 2295

Images (click to zoom)

The rat spinal cord section was stained with Rabbit antibody to BDNF (129-138): affinity purified (R-172-20), at a concentration of 2 µg/ml. Nerve terminals in the dorsal horn are labelled. Secondary detection with CY3 labelled antibodies. (Image courtesy of KJ Wong).

Section dorsal root ganglion from adult rat showing the presence of BDNF immunoreactivity in small diametre neurons and associated nerve fibres. Rabbit atnibody to BDNF (129-138): affinity purified (R-172-20), was used at 2 µg/ml. Secondary detection with CY3 labelled antibodies. (Image courtesy of KJ Wong).

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the MSDS

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