| Catalogue No. |
R-066-500 |
| Description |
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.
FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. |
| Batch No. |
See product label |
| Unit size |
500 µg |
| Antigen |
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species. |
| Other Names |
Brain-derived neurotrophic factor; Abrineurin; proBDNF; |
| Accession |
BDNF_HUMAN |
| Produced in |
Rabbit |
| Purity |
Protein G purified IgG |
| Applications |
IHC, ELISA, WB. A concentration of 1-10 µg/ml is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. |
| Cross-reactivity |
This antiserum is known to recognise rat, human and human BDNF. |
| Blast it |
If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| References |
1. A Acheson et al (1995) Nat. 74: 450-3
2. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91
3. XF Zhou et al (1996) Neurosci. 74: 945-53
4. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42
5. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9
6. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107
7. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9
8. Barde Y. A. et al (1989) EMBO J. 1: 549
9. Conner J et al. (1997) J. Neurosci. 17: 2295 |
