Rabbit antibody to rh BDNF: IgG

$190.00USD

Catalogue No.:	R-017-500
Batch No.:	See product label
Unit size:	500 µg
Antigen:	Recombinant human BDNF
Other Names:	Brain-derived neurotrophic factor, Abrineurin
Accession:	BDNF_HUMAN
Description:	BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Produced in:	NZ white rabbit
Purity:	Protein G purified IgG
Applications:	IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/ml for immunohistochemistry, Western blot or immunoblot, 0.1 µg/ml for ELISA and for inhibition of biological activity in vitro 1-10 µg/ml. Use neat for in vivo studies at 2-10 µg/ml (ED50). The optimal working dilution should be determined by the end user. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals.
Specificity:	Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot.
Cross-react:	This antiserum is known to react with BDNF from rat and human.
Blast it:	If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form:	Lyophilised
Reconstitution:	Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	12 months after purchase
References:	1. A Acheson et al (1995) Nat. 74: 450-3 2. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91 3. XF Zhou et al (1996) Neurosci. 74: 945-53 4. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42 5. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9 6. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107 7. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9 8. Barde Y. A. et al (1989) EMBO J. 1: 549 9. Conner J et al. (1997) J. Neurosci. 17: 2295
Related items:	R-081-50, R-082-50, R-066-500, R-083-100, R-084-100, R-O86-50, R-087-100, R-088-100, S-015-500, S-016-100

Images

Immunohistochemical staining for BDNF in rat dorsal root ganglion (DRG) using Rabbit antibody to rh BDNF: IgG (R-017-500) at an amount of 5 µg/ml. Neuron cell bodies are strongly stained for BDNF.

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