| Catalogue No. |
R-093-500 |
| Description |
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family. |
| Batch No. |
See product label |
| Unit size |
500 µg |
| Antigen |
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) |
| Other Names |
Beta-nerve growth factor |
| Accession |
P01139 NGF_MOUSE |
| Produced in |
NZ white rabbit |
| Purity |
Protein G purified IgG |
| Applications |
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF. Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF. The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems.
This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. |
| Cross-reactivity |
This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. |
| Blast it |
If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability. |
| Expiry Date |
12 months after purchase |
| Specific References |
1. Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54.
2. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95
|
| References |
1. Cassina P. et al (2005) Astrocyte activation by fibroblast growth factor-1 and motor neuron apoptosis: implications for amyotrophic lateral sclerosis. J Neurochem. 2005 Apr;93(1):38-46.
2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102.
3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75.
4. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240.
|
