Rabbit antibody to beta NGF: whole serum

$190.00USD

Catalogue No.:	R-085-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Other Names:	Beta-nerve growth factor
Accession:	NGF_MOUSE
Description:	FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µl/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. The optimal working dilution should be determined by the end user.
Specificity:	A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Cross-react:	This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
Blast it:	If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date:	12 months after purchase
References:	1. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240. 2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102. 3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75.
Related items:	R-094-50, R-093-500, S-051-50, S-052-500, S-050-250, R-134-100

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