| Catalogue No. |
R-165-100 |
| Description |
Clathrin is a major protein component of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice to either the plasma membrane or the trans-Golgi network. These specialised organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Clathrin localises to the cytoplasmic face of coated pit and vesicle membranes. |
| Batch No. |
See product label |
| Unit size |
100 µl |
| Antigen |
A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine. |
| Other Names |
Clathrin heavy chain; Clathrin heavy chain 1; CLH-17; CLTC |
| Accession |
CLH_RAT |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, WB. This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy i.e. a dilution range of 1: 100 to 1: 400.
For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
This antibody has been shown to be specific for clathrin. |
| Cross-reactivity |
Rat |
| Blast it |
If you would like to use this product in another speices other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. |
| References |
1. Kirchhausen T, et al. Proc. Natl. Acad. Sci. U.S.A. 84:8805-8809(1987).
2. Ter Haar E, et al. Cell 95:563-573(1998). |
