Rabbit antibody to AP-1 complex subunit gamma-1 (5-24): whole serum

$287.00USD

Catalogue No.	R-166-100
Description	Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
Batch No.	See product label
Unit size	100 µl
Antigen	A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Other Names	AP-1 complex subunit gamma-1, Adapter-related protein complex 1 subunit gamma-1, Gamma1-adaptin, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, GENE NAME: AP1G1, ADTG, CLAPG1
Accession	AP1G1_HUMAN
Produced in	NZ white rabbit
Purity	Whole serum
Applications	IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions are 1: 200 to 1: 2000 depending on tissue and detection method. For western blotting, 1: 1000 to 1: 4000 is recommended depending on detection method. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	This antibody has been shown to be specific for AP-1
Cross-reactivity	Rat and human
Blast it	If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form	Lyophilised
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent.
Expiry Date	12 months after purchase
References	1. Peyrard M, et al. Genomics 50:275-280(1998). 2. Takatsu H, et al. J. Biol. Chem. 273:24693-24700(1998).

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Rabbit antibody to AP-1 complex subunit gamma-1 (5-24): whole serum

Immunohistochemistry using DAB substrate showing intense staining in the rat airway epithelium using Rabbit antibody to AP-1 complex subunit gamma-1: whole serum (R-166-100) at a dilution of 1: 500. Secondary antibody was biotinylated goat and rabbit at a dilution of 1: 3000 followed by Vector ABC. This antigen has a similar distribution to clathrin, with which it associates.

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