Rabbit antibody to AP-1 complex subunit gamma-1 (5-24): whole serum

$190.00USD

Catalogue No.:	R-166-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Other Names:	AP-1 complex subunit gamma-1, Adapter-related protein complex 1 subunit gamma-1, Gamma1-adaptin, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, Clathrin assembly protein complex 1 gamma-1 large chain, GENE NAME: AP1G1, ADTG, CLAPG1
Accession:	AP1G1_HUMAN
Description:	Adaptins are important components of clathrin-coated vesicles transportiing ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called and adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-1 is a gamma-adaptin protein and belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions are 1: 200 to 1: 2000 depending on tissue and detection method. For western blotting, 1: 1000 to 1: 4000 is recommended depending on detection method. The optimal dilution should be determined by the end user.
Specificity:	This antibody has been shown to be specific for AP-1
Cross-react:	Rat and human
Blast it:	If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
Expiry Date:	12 months after purchase
References:	1. Peyrard M, et al. Genomics 50:275-280(1998). 2. Takatsu H, et al. J. Biol. Chem. 273:24693-24700(1998).
Related items:	R-165-100, R-167-100

Images

Rabbit antibody to AP-1 complex subunit gamma-1 (5-24): whole serum

Immunohistochemistry using DAB substrate showing intense staining in the rat airway epithelium using Rabbit antibody to AP-1 complex subunit gamma-1: whole serum (R-166-100) at a dilution of 1: 500. Secondary antibody was biotinylated goat and rabbit at a dilution of 1: 3000 followed by Vector ABC. This antigen has a similar distribution to clathrin, with which it associates.

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