| Catalogue No. |
R-170-100 |
| Description |
SOD3 is a member of the superoxide dismutase protein family. SODs are antioxidant enzymes that catalyse the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. SOD3 is thought to protect the brain, lungs and other tissues from oxidative stress. It is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A small percentage of SOD3 is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the extracellular matrix. |
| Batch No. |
See product label |
| Unit size |
100 µl |
| Antigen |
A synthetic peptide (WTGEDSAEPNSDSAEWIRD) corresponding to the amino acids 18-37 of human extracellular superoxide dismutase conjugated to diphtheria toxin has been used as the immunogen. |
| Other Names |
EC-SOD, GENE NAME: SOD3 |
| Accession |
SODE_HUMAN |
| Produced in |
NZ white rabbit |
| Purity |
Whole serum |
| Applications |
IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 2000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. This antibody stains prominently specific human airway (probably Clara cells) and alveolar type II cells and some tubular cells in the kidney. Other tissues have not yet been tested but the gene expression has also been reported in heart, pancreas and placenta. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
This antibody has been shown to be specific for extracellular superoxide dismutase protein. |
| Cross-reactivity |
Human |
| Blast it |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| References |
1. Hjalmarsson K, et al. Proc. Natl. Acad. Sci. U.S.A. 84:6340-6344(1987).
2. Folz R.J, et al. Genomics 22:162-171(1994). |
