Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum

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Home » Catalog » Antibodies » Autophagy » R-142-100

Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum

$245.00USD

Catalogue No.:	R-142-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
Other Names:	Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, MAP1 light chain 3-related protein. GENE NAME: GABARAPL2, FLC3A, GEF2.
Accession:	GBRL2_HUMAN GBRL2_MOUSE GBRL2_RAT
Description:	FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user.
Specificity:	IHC, WB and ELISA confirmed the specificity for GABARAP L2 (GABARAPL2).
Cross-react:	Human, rat. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	Six months after purchase
References:	1. Okazaki N. et al. Brain Res. Mol. Brain Res. 85:1-12(2000) 2. Xin Y. et al. Genomics 74:408-413(2001) 3. The MGC Project Team. Genome Res. 14:2121-2127(2004)

Images

Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum

Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.

	A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented.
	B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented.
	C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour).
	Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100.

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