| Catalogue No. |
R-142-100 |
| Description |
FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family. |
| Batch No. |
See product label |
| Unit size |
100 µl |
| Antigen |
A synthetic peptide (CVESAKIRAKYP) corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2). |
| Other Names |
Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 3-related protein; GABARAPL2; FLC3A; GEF2; |
| Accession |
GBRL2_HUMAN
GBRL2_MOUSE
GBRL2_RAT |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
IHC, WB and ELISA confirmed the specificity for GABARAP L2 (GABARAPL2). A 14kDa band, that corresponds to the molecular weight of GABARAPL2, is detected via western blot analysis. |
| Cross-reactivity |
Human, rat. Other species not yet tested. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
Six months after purchase |
| References |
1. Okazaki N. et al. Brain Res. Mol. Brain Res. 85:1-12(2000)
2. Xin Y. et al. Genomics 74:408-413(2001)
3. The MGC Project Team. Genome Res. 14:2121-2127(2004) |
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A. Confocal microscopy on immunofluorescently detected MPO (in green) in cytospin-isolated human white blood cells counter-stained with Hoechst Dye. Here, the merged picture is presented. |
|
B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were stained for GABARAP L2 (GABARAPL2) appearing in red counter-stained with Hoechst DyeHere. The merged picture is presented. |
|
C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP L2 (GABARAPL2) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP L2 (GABARAPL2) appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were counter stained with Hoechst Dye (blue colour). |
|
Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to GABARAP L2 (GABARAPL2): whole serum (R-142-100) at a dilution of 1:100. |
