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Rabbit antibody to GABARAP: whole serum

$297.00USD


Catalogue No. R-143-100
Description GABARAP is highly positively charged in its N-terminus and shares sequence homology with MAP1LC3 1A and 1B. This protein clusters neurotransmitter receptors (GABA(A) receptors) by mediating interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. TISSUE SPECIFICITY: Heart, brain, placenta, skeletal muscle, kidney and pancreas.
Batch No. See product label
Unit size 100 l
Antigen A synthetic peptide (FEKRRSEGEKIC) corresponding to the N-terminal of human GABARAP protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP.
Other Names Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46; GABARAP; FLC3B
Accession GBRAP_HUMAN
GBRAP_MOUSE
GBRAP_RAT
Produced in Rabbit
Purity Whole serum
Applications IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC, WB and ELISA confirmed the specificity for GABARAP.
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Wang H. et al. Nature. 397: 69-72 (1999)
2. Okazaki N. et al. Brain Res. Mol. Brain Res. 85:1-12(2000)
3. Hu R.-M. et al. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000)
4. Knight D. et al. Biol. Chem. 277:5556-5561(2002)
Images (click to zoom)
Rabbit antibody to GABARAP: whole serum Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1:100.
Rabbit antibody to GABARAP: whole serum A. Confocal microscopy on immunofluorescently detected GABARAP (in red) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1: 200, incubated for 1 h at room temperature, counter-stained with Hoechst Dye. Here, the merged picture is presented.
Rabbit antibody to GABARAP: whole serum B. Confocal microscopy on immunofluorescently detected MHC class II (in green) in cytospin-isolated human white blood cells. The cells were counter-stained with Hoechst Dye. The merged picture is presented.
Rabbit antibody to GABARAP: whole serum C is the merged picture of Figures A & B. Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to GABARAP: whole serum Confocal microscopy on immunofluorescently detected GABARAP (in red) in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1: 200, incubated for 1 h at room temperature.
Rabbit antibody to GABARAP: whole serum Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to GABARAP: whole serum Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1: 200, incubated for 1 h at room temperature. The cells were double-stained for GABARAP appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Rabbit antibody to GABARAP: whole serum Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to GABARAP: whole serum (R-143-100) at a dilution of 1:100.
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