| Catalogue No.: |
R-058-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (aa: 114-127) conjugated to diphteria toxid has been used as the immunogen. |
| Other Names: |
Persyn; Breast cancer-specific gene 1 protein; Synoretin; SR. Gena name: SNCG |
| Accession: |
SYUG_HUMAN |
| Description: |
FUNCTION: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. SIMILARITY: Belongs to the synuclein family. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHS, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. The optimal working dilution should be determined by the end user. |
| Specificity: |
Immunohistochemical/western blot anlysis indicate a high level of specificity for this antiserum for gamma synuclein. |
| Cross-react: |
This antiserum is known to react with human and rat gamma synuclein. |
| Blast it: |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchasse |
| References: |
1. Lundvig, et al Brain Res Mol Brain Res 134, 3-17 (Mar 24, 2005).
2. Bennett, Pharmacol Ther 105, 311-31 (Mar, 2005).
3. Vekrellis, et al., Mol Neurobiol 30, 1-21 (Aug, 2004).
4. Martin, et al., Protein Pept Lett 11, 229-37 (Jun, 2004).
5. Doherty, et al., Acta Neuropathol (Berl) 107, 169-75 (Feb, 2004).
6. Goedert, Curr Opin Genet Dev 11, 343-51 (Jun, 2001).
7. Saito, et al., J Neurol Sci 177, 48-59 (Aug 1, 2000).
8. Lucking, et al., Cell Mol Life Sci 57, 1894-908 (Dec, 2000).
9. Kahle, et al., Ann N Y Acad Sci 920, 33-41 (2000).
10. Clayton, et al., Trends Neurosci 21, 249-54 (Jun, 1998). |
| Related items: |
R-075-100, R-056-100, R-060-100, R-114-100, R-113-100, R-162-100, R-115-100, R-116-100, R-117-100, S-078-50, S-075-50, S-024-100, S-077-100, S-041-100, S-069-20, S-070-100, S-044-100, S-064-100 |
Images
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Immunohistochemical staining of gamma synuclein in rat brainstem using rabbit polyclonal to human gamma synuclein, catalogue number R-058-100. |
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Gamma synuclein immunoreactivity: Lane 1, recombinant human gamma synuclein imunoblotted with rabbit polyclonal to human gamma synuclein, catalogue number R-058-100; Lane 2, recombinant human beta synuclein immunoblotted with R-058-100; Lane 3, recombinant human alpha synuclein immunoblotted with R-058-100 |
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