| Catalogue No. |
R-171-100 |
| Description |
GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer. |
| Batch No. |
See product label |
| Unit size |
100 µl |
| Antigen |
A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine. |
| Other Names |
Extracellular glutathione peroxidase; GPx-P; GSHPx-3; GPx-3; Plasma glutathione peroxidase; GSHPx-P; GPX3; GPXP |
| Accession |
GPX3_HUMAN |
| Produced in |
Rabbit |
| Purity |
Whole serum |
| Applications |
IHC, WB. This is an extremely high titre antibody that detects 500 ng of peptide antigen on western blotting up to a dilution of 1: 500000. A dilution o f 1: 1000 to 1: 5000 is recommended for immunohistochemistry depending on detection method but end user will need to determine dilution to suit application. Antigen retrieval is recommended. For Western blotting it is recommended that several dilutions be tested in the range 1: 5000 to 1: 50000 although higher dilutions could be use if the antigen is in abundance in sample. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P). |
| Cross-reactivity |
Human and rat, other species have not yet been ested. |
| Blast it |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form |
Lyophilised |
| Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. |
| Expiry Date |
12 months after purchase |
| References |
1. Takahashi K, et al. J. Biochem. 108:145-148(1990).
2. Chu F.-F, et al. Blood 79:3233-3238(1992). |
