| Catalogue No.: |
R-155-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide corresponding to the N-terminal of human MAP1LC3 B (MAP1LC3B, APG8b) protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B (MAP1LC3B, APG8b) protein. |
| Other Names: |
Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B, MAP1A/1B light chain 3 B, MAP1 light chain 3-like protein 2, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, APG8b. GENE NAME: MAP1LC3B; MAP1ALC3. |
| Accession: |
MLP3B_HUMAN
MLP3B_MOUSE
MLP3B_RAT |
| Description: |
MAP1LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B is probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
|
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. |
| Specificity: |
IHC, WB and ELISA confirmed the specificity for MAP1LC3 B (MAP1LC3B, APG8b). |
| Cross-react: |
Human, rat. Other species not yet tested. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
| References: |
1. Difilippantonio S, et al. Eur. J. Cancer 39:1936-1947(2003).
2. Yang Y.-S, et al. Endocr. Relat. Cancer 10:621-627(2003).
3. He H, et al. J. Biol. Chem. 278:29278-29287(2003).
4. Ota T, et al. Nat. Genet. 36:40-45(2004).
5. Tanida I, et al. J. Biol. Chem. 279:36268-36276(2004).
6. Tanida I, et al. J. Biol. Chem. 279:47704-47710(2004).
7. Tanida I, et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004).
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| Related items: |
R-145-100, R-137-500, R-112-100, R-138-500, R-111-100, R-158-100, R-144-100, R-159-100, R-157-100, R-156-100, R-161-100, R-160-100, R-141-100, R-143-100, R-146-100, R-142-100, R-140-100 |
Images
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Western blot under reduceding condition on Olfactory cell line (Odora) lysate using Rabbit antibody to MAP1LC3 B (MAP1LC3B, APG8b): whole serum (R-155-100) at a dilution of 1:100. |
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Western blot under reduceding condition on 293 cell lysate using Rabbit antibody to MAP1LC3 B (MAP1LC3B, APG8b): whole serum (R-155-100) at a dilution of 1:100. |
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