| Catalogue No.: |
R-124-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide (CAQLRR IGDKVNLRQK) as part of mouse Noxa (aa: 75-90) conjugated to diphtheria toxoid |
| Other Names: |
PMAIP1, phorbol-12-myristate-13-acetate-induced protein 1; adult T cell leukemia-derived PMA-responsive, Immediate-early-response protein APR, PMA-induced protein 1. GENE NAME: Pmaip1; Noxa |
| Accession: |
NOXA_MOUSE |
| Description: |
The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line. |
| Produced in: |
NZ white rabbit |
| Purity: |
Whole serum |
| Applications: |
WB. A dilution of 1:1000 to 1:2000 is recommended for this application. The optimal dilution should be determined by the end user. |
| Specificity: |
Western blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum. |
| Cross-react: |
This antiserum cross-reacts with mouse. Not yet tested in other species. |
| Blast it: |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| References: |
1. Jansson, A.K., et al., Oncogene 22(30):4675-4678 (2003).
2. Hijikata, M., et al., J. Virol. 64(10):4632-4639 (1990). |
| Related items: |
R-123-100 |
