Rabbit antibody to recombinant EGFP: whole serum

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Home » Catalog » Antibodies » Reporter Genes » R-110-100

Rabbit antibody to recombinant EGFP: whole serum

$190.00USD

Catalogue No.:	R-110-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	Recombinant EGFP
Other Names:	Recombinant Enhanced Green Fluorescence Protein
Accession:	EGFP protein GFP_AEQVI
Description:	FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. SUBUNIT: Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. SIMILARITY: Belongs to the GFP family.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, Western blot. Recommended to be used at a dilution of 1:1000-1: 5000 for both applications. The optimal working dilution should be determined by the end user.
Specificity:	This antibody is known to react with EGFP confirmed by IHC and WB.
Blast it:	If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	12 months after purchase
References:	1. Chalfie, M., Tu, Y. et al (1994) Science 263, 802-805 2. Tsien, R. Y. (1998) Annu Rev Biochem 67, 509-544 3. Dardalhon, V. et al (1999) Hum Gene Ther 10, 5-14 4. Kain, S. R. (1999) DDT 4, 304-312
Related items:	G-002-100

PDF Data Sheet	- Click to download the Data Sheet
MSDS	- Click to download the Material Safety Data Sheet

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