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Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum

$297.00USD


Catalogue No. R-168-100
Description SOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Batch No. See product label
Unit size 100 l
Antigen A peptide (HEKADDLGKGGNEESTKTG) corresponding to the amino acids 121-139 of human superoxide dismutase (SOD1) conjugated to diphtheria toxin has been used as the immunogen.
Other Names Superoxide dismutase; SOD1;
Accession SODC_HUMAN
Produced in Rabbit
Purity Whole serum
Applications IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody has been shown to be specific for superoxide dismutase (SOD1) protein.
Cross-reactivity Human. Does not detect mouse and rat SOD1.
Blast it If you would like to use this product in another speices other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months after purchase
References 1. Jabusch J.R, et al. Biochemistry 19:2310-2316(1980).
2. Levanon D, et al. EMBO J. 4:77-84(1985).
Images (click to zoom)
Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum Immunohistochemical detection (DAB staining) of superoxide dismutase (SOD1) in paraffin embedded human brain tissue using Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum (R-168-100) at a dilution of 1: 100 incubated overnight. Secondary antibody was biotinylated goat anti-rabbit used at a dilution of 1: 3000 together with Vector ABC. This antibody labels neurons and some glial cells in human brain tissue. Other tissues have not yet been tested.
Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum Detection of SOD1 protein (16 kDa) in SH-SY5Y cell lysates with R-168-100. This antibody does not detect SOD1 in mouse and rat tissue samples. Proteins were separated by reduced SDS-PAGE, transferred to nitrocellulose membrane. Blots were incubated with R-168-100 at 1:1000 dilution using an anti-rabbit-HRP conjugate (1:10000) as secondary antibody.
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MSDS

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