Rabbit antibody to Tyrosine Hydroxylase (TH): whole serum

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Home » Catalog » Antibodies » Neurotransmitters & Enzymes » R-118-100

Rabbit antibody to Tyrosine Hydroxylase (TH): whole serum

$245.00USD

Catalogue No.:	R-118-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide (SPRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (61-77) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (30-46).
Other Names:	TH, Tyrosine hydroxylase, Tyrosine 3-monooxygenase, L-tyrosine hydroxylase, Tyrosine 3-hydroxylase
Accession:	TY3H_HUMAN TY3H_RAT
Description:	Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC. A dilution of 1:2000 to 1: 5000 is recommended for this application. The optimal working dilution should be determined by the end user. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labeling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals.
Specificity:	IHC on brain shows a pattern of staining specific for TH containing neurons.
Cross-react:	This antibody is known to react with rat. Cross reactivity with other species has not yet been tested.
Blast it:	If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
References:	1. Mallett, J. Trends in Pharmacological Science. 17(4): 129-135, 1996. 2. Haavik, J. et al. Mol. Neurobiology 16(3) :285-309, 1998. 3. Lewis DA, et al, Neuroscience 54: 477, 1993 4. Kumer S.C. et al. Journal of Neurochemistry, 67(2) :443-462, 1996. 5. Haycock, J. Anal. Biochemistry 181: 259-266, 1989. 6. Haycock, J. Anal. Biochemistry 208: 397-399, 1993. 7. Renfroe, J.B., et al. Brain Res. Bull. 13: 109-126, 1984. 8. Xu, Z et a.l Neurosci. 82(3): 727, 1998

Images

Rabbit antibody to Tyrosine Hydroxylase (TH): whole serum

Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.

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Rabbit antibody to Tyrosine Hydroxylase (TH): whole serum

brilliant staining of TH in rat brain ..