Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum

$267.00USD

Catalogue No.:	R-152-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	Extracellular domain of glycosylated human TrkA protein produced in CHO cells was used as the immunogen.
Other Names:	Tropomyosin-related kinase receptor, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1 transforming tyrosine kinase protein, p140-TrkA, Trk-A, GENE NAME: NTRK1, TRK.
Accession:	NTRK1_HUMAN
Description:	TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.
Produced in:	NZ white rabbit
Purity:	Whole serum
Applications:	IHC, 1-site ELISA (1:5000 dilution). A dilution of 1:1000 is recommended for IHC. The optimal working dilution should be determined by the end user.
Specificity:	Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons.
Cross-reactivity:	Reacts with human, rat and mouse TrkA. Other species not yet tested.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
References:	1. Zhang FX, et al. (2005) Brain Res 16; 1062 (1-2) pp. 92-100. 2. Gearhart DA, et al. (2006) J Neurosci Methods. 150(2) pp. 159-173.

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Rabbit antibody to the Tyrosine Kinase Receptor A (TrkA): whole serum

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat spinal cord (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat trigeminal nerve (free floating cryostat section) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

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