| Catalogue No. |
R-1009-100 |
| Description |
Excitatory amino acid transporter 1 (EAAT1) is a glutamate transporter (GluT). It rapidly removes L-glutamate from the synaptic cleft which is essential for terminating the postsynaptic action of glutamate. EAAT1 is also known as high affinity sodium-dependent glutamate/aspartate transporter 1 (GLAST1). EAAT1 is highly expressed in cerebellum but is also found in the frontal cortex, hippocampus and basal ganglia. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
A synthetic peptide (ENEMKKPYQLIAQDNE) corresponding to a region 516-531 from the C-terminus of human EAAT1/GLAST1. |
| Other Names |
SLC1A3; GLAST; GLT-1; |
| Accession |
P43003 EAA1_HUMAN; P24942 EAA1_RAT |
| Produced in |
Rabbit |
| Purity |
Affinity purified on antigen column |
| Applications |
Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human EAAT1 has a predicted length of 542 residues and a MW of 60 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example. |
| Form |
Lyophilized |
| Reconstitution |
Each vial contains 5mg of BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal and 0.05mg NaN3. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
