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Rabbit polyclonal antibody to Endomorphin-2 (1-4): Affinity purified

$297.00USD


Catalogue No. R-1496-50
Description Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Batch No. See product label
Unit size 50 g
Antigen Synthetic Endomorphin-2 (YPFF) peptide conjugated to BSA. Selectivity towards Endomorphin-2 was achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin-1.
Sequence YPFF
Other Names EM 2;
Produced in Rabbit
Applications A dilution of 5-10 g/ml is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 g/ml is recommended for immunofluorescence. . Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Species Against Human
Antibody Against Endomorphin-2
Cross-reactivity Human; mouse; rat.
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between proteins. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Blast URL Click here
Form Lyophilised with BSA
Appearance White powder
Reconstitution Reconstitute in 0.05 ml of PBS (pH 7.4) to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability and at 4C with an appropriate antibacterial agent. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to Endomorphin-2 (1-4): Affinity purified Immunohistochemical staining in rat ventral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain crystostat sections (10 m) were incubated overnight at 4C with Rabbit polyclonal antibody to Endomorphin-2 (10 g/ml) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

PDF Data Sheet

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MSDS

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