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Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP): Whole serum

$297.00USD


Catalogue No. R-1374-50
Description GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Batch No. See product label
Unit size 50 l
Antigen Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Antibody Type Antiserum
Other Names Astrocyte; Glial fibrillary acidic protein; GFAP;
Accession P14136 GFAP_HUMAN; Q28115 GFAP_BOVIN;
Produced in Rabbit
Applications Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:50,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB.
Antibody Against Glial Fibrillary Acidic Protein
Cross-reactivity Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Blast URL Click here
Form Lyophilised
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
References 1. Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989).
2. Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990).
2. Isaacs A, et al. Genomics 51:152-154(1998).
3. Ota T, et al. Nat. Genet. 36:40-45(2004).
4. Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002).
5. Singh R, et al. Genomics 82:185-193(2003).
6. Brenner M, et al. Nat. Genet. 27:117-120(2001).
7. Brockmann K, et al. Eur. Neurol. 50:100-105(2003).
8. Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003).
9. Sawaishi Y, et al. Neurology 58:1541-1543(2002).
10. Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Images (click to zoom)
Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP): Whole serum Mixed neuron-glial cultures stained with Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red channel) and Chicken polyclonal antibody to Vimentin C-1409-50 (green channel). The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and GFAP, so appearing golden, or predominantly GFAP, in which case they appear red. Blue is nuclear DNA stain.
Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP): Whole serum View of a thin section of adult rat cerebellum stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (green), Rabbit polyclonal antibody to GFAP R-1374-50 (red) and DNA (blue). The image shows the molecular layer (outside of lobe) and granular layer; blue since its full of small neurons and the white matter in the middle.
Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP): Whole serum A view of neonatal rat brain cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (red) and with Rabbit polyclonal antibody to GFAP R-1374-50 (green). These two proteins are found only in non-neuronal cells so you can\'t see any neurons, except for their nuclei in blue. Maturish astrocytes have only GFAP so appear green or have a mix of both proteins, so appear yellow. Some cells only have the vimentin (immature astrocytes, microglia and fibroblasts) and so appear red.
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MSDS

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