| Catalogue No. |
R-1569-100 |
| Description |
Corticotropin-releasing hormone (CRH) is a 41 amino acid peptide derived from a preprohormone. CRH is secreted from the hypothalamus and is also synthesized in peripheral tissues such as T lymphocytes and is highly expressed in the placenta. CRH regulates the release of corticotropin from the pituitary gland. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
A synthetic peptide corresponding to a region (1-17 aa) from the N-terminus of Corticotropin-releasing hormone. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names |
Corticotropin-releasing hormone; Corticotropin-releasing factor; CRF; CRH; Corticoliberin; |
| Accession |
P06850 CRF_HUMAN; |
| Produced in |
Rabbit |
| Purity |
Affinity purified on antigen column |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/ml is recommended for WB. Human CRH preprohormone has a predicted length of 196 residues and MW of 21 kDa. A concentration of 0.5-1.0 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Form |
Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Reconstitution |
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material. |
| Storage |
After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles |
| Expiry Date |
12 months after purchase. |
