| Catalogue No. |
R-1599-100 |
| Description |
Cyclooxygenase (COX) is an important enzyme in the conversion of arachidonic acid to Prostaglandin H2, the precursor of the series-2 prostanoids. At least 3 splice variants have been found (COX-1, COX-2 and COX-3). COX-1 is constitutively expressed in most cell types and is induced as part of the inflammatory response by cytokines as well as by growth factors such as EGF and PDGF. |
| Batch No. |
See product label. |
| Unit size |
100 µg |
| Antigen |
A synthetic peptide corresponding to a region (35-52 aa) from human Cyclooxygenase-1. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names |
Prostaglandin G/H synthase 1; Cyclooxygenase-1; COX-1; Prostaglandin-endoperoxide synthase 1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; PTGS1;COX1; |
| Accession |
P23219 PGH1_HUMAN; |
| Produced in |
Rabbit |
| Purity |
Affinity purified on antigen column |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5µg/ml is recommended for WB. Human COX-1 (long) has a predicted length of 599 residues and MW of 69 kDa. A concentration of 0.5-1.0 µg/ml is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Cross-reactivity |
Human (WB, IHC); rat (WB); mouse (WB); |
| Form |
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 added as preservative |
| Reconstitution |
Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles |
| Expiry Date |
12 months after purchase |
