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Rabbit polyclonal antibody to human D(1A) dopamine receptor (425-437): Affinity purified

$297.00USD


Catalogue No. R-1139-100
Description D(1A) dopamine receptor (DRD1) is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. DRD1 is one of five receptors for dopamine. DRD1 stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. At least 2 isoforms exist arising from alternate transcription initiation sites.
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide (DYDTDVSLEKIQP) corresponding to a region (425-437) from human D(1A) dopamine receptor. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names DRD1;
Accession P21728 DRD1_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Western Blotting (WB). A concentration of 1.0 g/ml is recommended for WB. Human DRD1 has a predicted length of 446 residues and a MW of 49 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB against the antigen.
Cross-reactivity Human; rat; predicted to react with mouse due to sequence homology;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles
Expiry Date 12 months after purchase
Images (click to zoom)
Rabbit polyclonal antibody to human D(1A) dopamine receptor (425-437): Affinity purified Western blot analysis of rabbit polyclonal antibody to human D(1A) dopamine receptor (1μg/ml) in rat brain tissue lysate. Secondary HRP conjugated goat anti-rabbit IgG antibody (working dilution: 1:3000-10000).

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MSDS

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