| Catalogue No. |
R-1469-100 |
| Description |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing the first step of the glycolysis pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
A synthetic peptide corresponding to a region (30-44 aa) from the N-terminus of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). |
| Antigen Location |
30-44 |
| Other Names |
Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; GAPD; |
| Accession |
P04406 G3P_HUMAN; |
| Produced in |
Rabbit |
| Applications |
Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/ml is recommended for WB. Human GAPDH (precursor) has a predicted length of 335 amino acids and MW of 36 kDa. A concentration of 1.0 µg/ml is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0 µg/ml is also recommended for IHC in frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Antibody Against |
Glyceraldehyde-3-phosphate dehydrogenase |
| Cross-reactivity |
Human; rat; mouse; |
| Blast URL |
Click here |
| Form |
Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Appearance |
White powder |
| Reconstitution |
Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date |
12 months after purchase |
