| Catalogue No.: |
R-963-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µg |
| Antigen: |
A synthetic peptide (YSKVYFRGQSCNNLPLSHKVYMRNSKY) corresponding to a region (192-218) from human FAS ligand (Fas-L). To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names: |
CD95L; CD178 antigen; FASLG; TNFSF6; APT1LG1; |
| Accession: |
P48023 TNFL6_HUMAN |
| Description: |
The FAS ligand (Fas-L) belongs to the tumor necrosis factor family. The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing. |
| Produced in: |
Rabbit |
| Purity: |
Affinity purified on antigen column |
| Applications: |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/ml is recommended for WB. Human Fas-L has a predicted length of 281 residues and a band size between 31 - 40 kDa with glycosylation. A concentration of 1.0-2.0 µg/ml is recommended to detect Fas-L in formalin fixed and paraffin embedded tissues. |
| Specificity: |
The specificity of this antibody has been confirmed by WB and IHC against the immunogen. |
| Cross-reactivity: |
Human; mouse; rat |
| Blast it: |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form: |
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 added as preservative |
| Reconstitution: |
Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage: |
At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date: |
12 months after purchase |
