| Catalogue No. |
R-1076-100 |
| Description |
Placenta growth factor belongs to the PDGF/VEGF growth factor family. It is a secreted N-glycosylated protein with roles in angiogenesis and endothelial cell growth. At least 3 isoforms are produced by alternative splicing; PIGF-1, PIGF-2 and PIGF-3. |
| Batch No. |
See product label |
| Unit size |
100 µg |
| Antigen |
A synthetic peptide (EVEVVPFQEVWGRSYC) corresponding to a region (37-52) from human Placenta growth factor. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names |
PlGF; PGF; PGFL; PLGF; |
| Accession |
P49763 PLGF_HUMAN; |
| Produced in |
Rabbit |
| Purity |
Affinity purified on antigen column |
| Applications |
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/ml is recommended for WB. Human placenta growth factor (isoform PIGF-3) has a predicted length of 221 residues and MW of 25 kDa. A concentration of 2.0 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Cross-reactivity |
Human; mouse; rat; |
| Blast it |
If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form |
Liquid (0.5ml) in 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4 |
| Reconstitution |
The liquid formulation should be diluted in PBS (pH 7.4) |
| Storage |
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles |
| Expiry Date |
12 months after purchase |
