Rabbit polyclonal antibody to Internexin alpha: Whole serum

$287.00USD

Catalogue No.	R-1379-50
Description	Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Batch No.	See product label
Unit size	50 µl
Antigen	Recombinant alpha-internexin expressed and purified from E. coli
Antibody Type	Antiserum
Other Names	67 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;
Accession	Q16352 AINX_HUMAN; P23565 AINX_RAT;
Produced in	Rabbit
Applications	Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species.
Antibody Against	Internexin alpha
Cross-reactivity	Hu, Rat, Ms, Fel, and other mammals
Blast URL	Click here
Form	Lyophilised
Appearance	White powder
Reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

Images (click to zoom)

Rabbit polyclonal antibody to Internexin alpha: Whole serum

Mixed neuron-glial cultures stained with Rabbit polyclonal antibody to Internexin alpha R-1379-50 (red) and Chicken polyclonal antibody to peripherin C-1399-50 (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while Peripherin antibody binds to only a subset of neurons.

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MSDS	- Click to download the MSDS