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Rabbit polyclonal antibody to ATP synthase-coupling factor 6, mitochondrial: Affinity purified

$297.00USD


Catalogue No. R-1766-100
Description Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. (Uniprot: P18859).
Batch No. See product label
Unit size 100 g
Antigen A synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
Other Names GENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
Accession P18859 ATP5J_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Western blot, 0.1-0.5g/ml, cell lysates. Predicted size: 12-15kDa , reduced conditions. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB.
Cross-reactivity Human and rat. The antibody is also expected to work on mouse because of protein homology.
Form Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 l of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C up to six months. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase (lyophilized)
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MSDS

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