||Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. (Uniprot: P18859).
||See product label
||A synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
||GENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
||Affinity purified on antigen column
||Western blot, 0.1-0.5µg/ml, cell lysates. Predicted size: 12-15kDa , reduced conditions. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB.
||Human and rat. The antibody is also expected to work on mouse because of protein homology.
||Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
||Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C up to six months. Avoid freeze-thaw cycles.
||12 months after purchase (lyophilized)