||Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins.
||See product label
||Saponaria officinalis; Common soapwort; ribosome inactivating protein saporin-6; SAP-6; SO-6; rRNA N-glycosidase
||Purified from seeds of the plant Saponaria officinalis.
||Only qualified personnel should handle this product. Extreme precaution must be employed when handling the toxin. Wear lab coat, gloves and safety glasses. The toxin must not come into contact with open wounds. Practice good laboratory technique to avoid auto-exposure/auto-injection! Wash thoroughly any part of the body exposed to the toxin. Contaminated materials should be exposed to 300 mM NaOH or autoclaved.
||95% by SDS-PAGE and Western Blot.
||Upon introduction into the cells, saporin will arrest the protein synthesis by damaging the ribosmes.
||Saporin may be reconstituted in the buffer of choice for example PBS.
||Aliquot and keep at -20ºC for long-term storage. For short term keep at 4ºC. Avoid repetitive freeze/thaw cycles.
||Stirpe, F. et al (1983) Biochem J 216, 617-625