| Catalogue No.: |
S-008-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
Recombinant human basic FGF |
| Other Names: |
Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, bFGF, Prostatropin. Gene name: FGF2; FGFB |
| Accession: |
FGF2_HUMAN |
| Description: |
Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. FUNCTION: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. SIMILARITY: Belongs to the heparin-binding growth factors family. |
| Produced in: |
Sheep |
| Purity: |
Whole serum |
| Applications: |
IHC, WB. Recommended to be used at a dilution of 1: 1000 to 1:2000 for both applications. The optimal working dilution should be determined by the end user. |
| Specificity: |
A high level of specificity for bFGF was shown by immunohistochemistry for this antiserum. |
| Cross-reactivity: |
This antibody is known to react with human, mouse and rat basic FGF. |
| Blast it: |
If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
| References: |
1. Abraham, et al. (1986) Science. 233(4763):545-8
2. Kurokawa, et al. (1987) FEBS Lett. 213(1):189-94
3. Bieger, et al., (1995) J Neurochem 64, 1521-7
4. Takayama, et al., (1995)Nat Med 1, 53-8.
5. Eckenstein (1994) J Neurobiol 25, 1467-80. (1994)
6. Abraham, et al., (1986) Embo J 5, 2523-8
7. English, et al., (1995)Dev Biol 169, 57-64. |
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