||BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
||See product label
||Recombinant human BDNF
||Brain-derived neurotrophic factor; Abrineurin; proBDNF;
||Protein G purified IgG
||IHC, ELISA, WB, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/ml for immunohistochemistry, ELISA, Western blot or inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA.
||Known to react with BDNF from rat and human.
||If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
||Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
||12 months after purchase
||1. Hayashida K., Eisenach J.C.(2010) Spinal alpha2-adrenoceptor-mediated analgesia in neuropathic pain reflects brain-derived nerve growth factor and changes in spinal cholinergic neuronal function Anesthesiology. 2010 Aug;113(2):406-12.
2. Geremia N.M. et al (2010) Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons Exp Neurol. 2010 May;223(1):128-42.
||1. Vissio P.G. et al (2008) Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes) Tissue Cell. 2008 Aug;40(4):261-70.
2. A Acheson et al (1995) Nat. 74: 450-3
3. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91
4. XF Zhou et al (1996) Neurosci. 74: 945-53
5. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42
6. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9
7. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107
8. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9
9. JA Coull et al (2005) Nature. Dec 15;438(7070):1017-21.
10. C Gomes et al (2013) J Neuroinflammation. Jan 30;10:16.