| Catalogue No.: |
S-045-50 |
| Batch No.: |
See product label |
| Unit size: |
50 µg |
| Antigen: |
A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat. |
| Other Names: |
Proto-oncogene protein cFOS |
| Accession: |
FOS_HUMAN
Q4FDN1_RAT
FOS_MOUSE
Q56UN0_PHOSU
Q5XQC6_FELCA |
| Description: |
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain |
| Produced in: |
Sheep |
| Purity: |
Affinity purified |
| Applications: |
IHC, WB. A concentration of 1 -5 µg/ml is recommended. The optimal dilution should be determined by the end user. |
| Specificity: |
This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. |
| Cross-react: |
This antiserum is known to react with rat, rabbit and hamster cFOS. |
| Blast it: |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 50 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
| References: |
1. Vanstraaten et al (1983) Proc. Natl. Acad. Sci. 80: 3183
2. Minson J. et al. (1994) Brain Res. 646: 44-52 |
