| Catalogue No.: |
S-061-100 |
| Batch No.: |
See product label |
| Unit size: |
100 µl |
| Antigen: |
A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa: 354-367) conjugated to diphtheria toxoid has been used as the immunogen. |
| Other Names: |
Gap junction gamma-1 protein, Gap junction alpha-7 protein, Cx45. GENE NAME: GJC1, GJA7 |
| Accession: |
CXA7_HUMAN |
| Description: |
Connexin-45 is a component of gap junctions, which are composed of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily. Alternatively spliced isoforms have been described. |
| Produced in: |
Sheep |
| Purity: |
Whole serum |
| Applications: |
Immunofluorescence, Western blot. A dilution of 1:10000 to 1: 20000 is recommended for Immunofluorescence and 1:20000 to 1:50000 for western blot. The optimal working dilution should be determined by the end user. |
| Specificity: |
Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47 kDa which is abolished by preincubation with the immunising peptide. No such band is detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40. |
| Cross-react: |
This antiserum reacts to human Connexin-45. |
| Blast it: |
If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different speices and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this. |
| Form: |
Lyophilised |
| Reconstitution: |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage: |
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date: |
12 months after purchase |
| References: |
1. Rummery et al. (2002). ATVB 22, 1429-1432 |
