Sheep antibody to SUMO-1 (6-21): whole serum

$190.00USD

Catalogue No.:	S-064-100
Batch No.:	See product label
Unit size:	100 µl
Antigen:	A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Other Names:	Small ubiquitin-related modifier 1, Ubiquitin-like protein SMT3C, SMT3 homolog 3, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, GAP-modifying protein 1, GMP1, Sentrin. GENE NAME: SUMO1; SMT3C; SMT3H3; UBL1
Accession:	SUMO1_HUMAN
Description:	SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PML, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Produced in:	Sheep
Purity:	Whole serum
Applications:	IHC, WB. A dilution of 1:2000 to 1:4000 is recommecnded for immunohistochemistry and 1:4000 to 1:8000 for western blot. The optimal dilution should be determined by the end user. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used.
Specificity:	This antiserum recognises human SUMO-1 and not ubiquitin.
Cross-react:	This antiserum is known to cross react with rat and human SUMO-1.
Blast it:	If you would like to use this product in another species other than those specified here, or to see the shared identity between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form:	Lyophilised
Reconstitution:	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage:	After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date:	12 months after purchase
References:	1. Yang, S.H. et al. Mol. Cell 13(4):611-617 (2004). 2. Ohshima, T. et al. J. Biol. Chem. 278(51):50833-50842 (2003). 3. Bailey, D. et al. J. Biol. Chem. 279(1):692-703 (2004). 4. Ling, Y. et al. Nucleic Acids Res. 32(2):598-610 (2004). 5. Pountney, D.L. et al. Exp. Neurol. 184(1):436-446 (2003).
Related items:	R-115-100, S-035-100

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