Application DetailsWestern blotting (1:5,000-1:10,000), Immunocytochemistry (1:5,000-1:10,000) and Immunohistochemistry (1:5,000-1:10,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen DescriptionFull-length recombinant human protein
Purity DescriptionIgY fraction
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMay participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Left: Mixed neuron-glial cell cultures stained with chicken anti-CNP (red) and mouse anti-GFAP antibody (M-1375-100) by Immunocytochemistry. The CNP antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue: Nuclear DNA stain, which reveals the presence of neurons which do not express either CNP or GFAP. Right: Western Blot analysis of rat brain tissue homogenate with chicken anti-CNP antibody (1:5,000). One specific band at ~48 kDa is detected corresponding to the molecular weight of CNP.
Left: Rat cerebellum section stained with chicken anti-CNP (green, 1:2,000) and rabbit anti-NF-H antibody (red, 1:10,000). IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The CNP antibody stains myelin and oligodendrocytes, cells that create the myelin sheath around axons. The NF-H antibody labels the heavily phosphorylated axonal forms of NF-H which are localized in large projection axons. Right: Western blot analysis of spinal cord tissue homogenates using chicken anti-CNP (red, 1:5,000). [2] mouse, [3] rat and [4] cow. Doublet bands at 46 and 48 kDa correspond to CNP isotypes. The blot was simultaneously probed with mouse anti-internexin antibody (green).
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