1800 605-5127
+61 (0)8 8352 7711
A2 Beta casein (A2), Bovine, ELISA assay
As low as
US$437.00
Only %1 left
Catalog Number
BEK-2244
- Product Name A2 Beta casein (A2), Bovine, ELISA assay
- Product Description β-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are β-Casein A1 and β-Casein A2. The two types of β-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 β-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 β-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine β-Casein polyclonal capture antibody, a chicken anti-bovine A2 β-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 β-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 β-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
- Alternative Names CSN2; Bovine A2 Beta Casein; A2;
- Application(s) ELISA
- Specificity Bovine A2 beta-casein
- Species Reactivity Bovine
- Immunogen Description Purified bovine beta Casein and peptide specific peptides for A2
- Range 3.1 - 200 ng/mL
- Sensitivity This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
- Regulatory Status For research use only.
Product Info
- Product Description β-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are β-Casein A1 and β-Casein A2. The two types of β-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 β-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 β-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine β-Casein polyclonal capture antibody, a chicken anti-bovine A2 β-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 β-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 β-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
-
Related Products
A1 Beta casein (A1), Bovine, ELISA assay
Casein, A2 beta, Chicken Polyclonal Antibody
Casein, A1 beta, Chicken Polyclonal Antibody
Casein, bovine beta A1, Purified Protein
Casein, bovine beta A2, Purified Protein
Beta casein protein, Rabbit Polyclonal Antibody
- Application(s) ELISA
- Application Details ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target A2 Beta casein (A2)
- Specificity Bovine A2 beta-casein
- Target Cross-Reactivity (ELISA) No cross-reactivity is observed with bovine A1 beta-Casein
- Target Host Species Bovine
- Species Reactivity Bovine
- Immunogen Description Purified bovine beta Casein and peptide specific peptides for A2
- Range 3.1 - 200 ng/mL
- Sensitivity This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
- Sample Type Bovine Milk
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt unopened.
- Alternative Names CSN2; Bovine A2 Beta Casein; A2;
- Uniprot Number P02666
- Uniprot Number/Name P02666 (CASB_BOVIN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8ºC (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
