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Adiponectin (ACRP30), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2120
- Product Name Adiponectin (ACRP30), Human, ELISA assay
- Product Description The human Adiponectin Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human Adiponectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Adiponectin concentration are added to these wells and allowed to complex with the bound Adiponectin antibody. A biotinylated anti-human Adiponectin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Adiponectin present in the samples. The purpose of this kit is for the quantitative detection of Human Adiponectin in culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Adipocyte; C1q and collagen domain-containing protein; ADIPOQ; Adipocyte complement-related 30 kDa protein; ACRP30; ACDC; ACRP30; APM1; GBP28;
- Application(s) ELISA
- Specificity Human Adiponectin
- Species Reactivity Human
- Immunogen Description Assay standard and immunogen is recombinant human adiponectin amino acids 19-244 expressed in and purified from a mammalian cell expression system.
- Range 1.56 ng/mL - 100 ng/mL
- Sensitivity < 60 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Adiponectin Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human Adiponectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Adiponectin concentration are added to these wells and allowed to complex with the bound Adiponectin antibody. A biotinylated anti-human Adiponectin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Adiponectin present in the samples. The purpose of this kit is for the quantitative detection of Human Adiponectin in culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Adiponectin (ACRP30) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Adiponectin (ACRP30)
- Specificity Human Adiponectin
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Assay standard and immunogen is recombinant human adiponectin amino acids 19-244 expressed in and purified from a mammalian cell expression system.
- Range 1.56 ng/mL - 100 ng/mL
- Sensitivity < 60 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Adipocyte; C1q and collagen domain-containing protein; ADIPOQ; Adipocyte complement-related 30 kDa protein; ACRP30; ACDC; ACRP30; APM1; GBP28;
- Uniprot Number Q15848
- Uniprot Number/Name Q15848 (ADIPO_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
