Left: Fluorescent staining of rat cerebellum section by Immunohistochemistry with chicken antibody to alpha-internexin (1:5,000, red), and co-stained with mouse antibody to MBP (M-1384-50, 1:5,000, green). IHC Method: Following transcardial perfusion with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons. Right: Western blot analysis of alpha-internexin expression in tissue lysates.  protein standard,  rat brain,  rat spinal cord,  mouse brain,  mouse spinal cord,  cow spinal cord,  pig spinal cord. The antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa, with some variability among different species. Antibody dilution: 1:10,000.