SpecificityThe specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Species ReactivityCat, Human, Mouse, Other Mammals (Predicted), Rat
Immunogen DescriptionRecombinant rat alpha-internexin expressed and purified from E. coli
Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAlpha-internexin (Alpha-Inx)
SpecificityThe specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Target Host SpeciesRat
Species ReactivityCat, Human, Mouse, Other Mammals (Predicted), Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionRecombinant rat alpha-internexin expressed and purified from E. coli
Purity DescriptionIgY
FormatLyophilized, without preservatives.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNeurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Mixed neuron glia cultures stain with Chicken polyclonal antibody to Internexin alpha C-1377-50 (red) and counterstained with Rabbit polyclonal antibody to Coronin 1a: Whole serum, R-1335-50 (green) which is an excellent marker of microglia and lymphocytes. This internexin antibody is an excellent marker of neuronal processes in these cultures.
Left: Fluorescent staining of rat cerebellum section by Immunohistochemistry with chicken antibody to alpha-internexin (1:5,000, red), and co-stained with mouse antibody to MBP (M-1384-50, 1:5,000, green). IHC Method: Following transcardial perfusion with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons. Right: Western blot analysis of alpha-internexin expression in tissue lysates. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, [7] pig spinal cord. The antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa, with some variability among different species. Antibody dilution: 1:10,000.
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