Application DetailsWestern blotting (1:1,000 - 1:2,000), Immunocytochemistry (1:1,000 - 1:2,000) and Immunohistochemistry (1:1,000 1: 2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAlpha synuclein
SpecificityHuman, reacts with human, horse, cow, pig, chicken, rat, mouse.
Target Host SpeciesHuman
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Pig, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionFull length human protein with the epitope from amino acids 61-95
Purity DescriptionAffinity purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and 5mM sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNon-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Scientific BackgroundMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Ref: uniprot.org.
Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.
Left: Analysis of alpha-synuclein expression in mixed rat neuron-glial cultures by Immunocytochemistry. The alpha-synuclein antibody (red) stains vesicular structures the perikarya and processes of the neurons. The antibody reveals that some of the neuronal perikarya contain more alpha-synuclein than others. Green: MAP2, Blue: DNA. Right: Detection of alpha-synuclein monomer (~15 kDa) in crude rat brain extract by Western Blotting.
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